a:5:{s:8:"template";s:12592:"<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="utf-8"/>
<title>{{ keyword }}</title>
<meta content="text/html; charset=utf-8" http-equiv="Content-Type"/>
<meta content="width=device-width,initial-scale=1.0" name="viewport"/>
<link href="http://fonts.googleapis.com/css?family=Yanone+Kaffeesatz:400,700" rel="stylesheet" type="text/css"/>
<link href="http://fonts.googleapis.com/css?family=Oswald" rel="stylesheet" type="text/css"/>
<style rel="stylesheet" type="text/css">@charset "utf-8";body,div,footer,form,h1,h2,h3,header,html,img,li,nav,p,span,time,ul{margin:0;padding:0;border:0;outline:0;font-size:100%;vertical-align:baseline;background:0 0}body{line-height:1}footer,header,nav{display:block}nav ul{list-style:none}a{margin:0;padding:0;font-size:100%;vertical-align:baseline;background:0 0}a{margin:0;padding:0;font-size:100%;vertical-align:baseline;background:0 0}input{vertical-align:middle}#sb-site,.sb-slidebar,body,html{margin:0;padding:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}body,html{width:100%;overflow-x:hidden}html{height:100%}body{min-height:100%;height:auto;position:relative}#sb-site{width:100%;position:relative;z-index:1}.sb-slidebar{height:100%;overflow-y:auto;position:fixed;top:0;z-index:0;display:none;background-color:#fff;-webkit-transform:translate(0)}.sb-right{right:0}.sb-slidebar{width:30%}@media (max-width:480px){.sb-slidebar{width:70%}}@media (min-width:481px){.sb-slidebar{width:55%}}@media (min-width:768px){.sb-slidebar{width:40%}}@media (min-width:992px){.sb-slidebar{width:30%}}@media (min-width:1200px){.sb-slidebar{width:20%}}#sb-site,.sb-slidebar{-webkit-transition:-webkit-transform .4s ease;-moz-transition:-moz-transform .4s ease;-o-transition:-o-transform .4s ease;transition:transform .4s ease;-webkit-transition-property:-webkit-transform,left,right;-webkit-backface-visibility:hidden} @font-face{font-family:'Yanone Kaffeesatz';font-style:normal;font-weight:700;src:url(http://fonts.gstatic.com/s/yanonekaffeesatz/v14/3y9I6aknfjLm_3lMKjiMgmUUYBs04aUXNxt9gW2LIftoEdKZd2GP.ttf) format('truetype')}body{margin:0;font-family:'Century Gothic',Helvetica,Arial,Sans-serif;font-size:14px;line-height:20px;text-align:left;vertical-align:top;background:#fff;color:#000}body,form,h1,h2,h3,p{margin:0;padding:0}h1{font-size:24px;padding:0 0 10px 0}a:link,a:visited{text-decoration:none;color:#ef717d}a:hover{color:#f28b2e}p{padding:0 0 10px}.clear{clear:both}#sb-site{}#wrapper{background:#fff;margin:0 auto 0 auto;max-width:1000px;text-align:left;width:100%}#innerWrap{padding:0 30px;width:auto}#mainHd{min-height:163px;width:100%}#mainHd h1{float:left;height:163px;margin:0;text-indent:-9999px;width:234px}#mainHd h1 a{display:block;height:163px;width:234px}#mainHd .menu{border-top:6px double #999b9a;border-bottom:6px double #999b9a;float:right;font-family:'Yanone Kaffeesatz';font-size:44px;font-weight:700;line-height:44px;list-style:none;margin:25px 0 10px;padding:5px 0;max-width:706px;text-transform:uppercase;width:auto}#mainHd .menu li{float:left;list-style:none;position:relative}#mainHd .menu li a{padding:0 12px}#mainHd .menu li ul{display:none;position:absolute;top:44px;left:0;width:180px;background:#fff;box-shadow:0 0 4px #606060;z-index:99}#mainHd .menu li:hover ul{display:block}#mainHd .menu li ul li{float:none;font-size:24px;line-height:24px;margin:0;padding:0}#mainHd .menu li ul li a{display:block;padding:10px 0 10px 15px}#mainHd .menu li:nth-child(6n-5) a{color:#ef7163}#mainHd .menu li:nth-child(6n-5) a:hover{color:#999b9a}#mainHd .menu li:nth-child(6n-4) a,#mainHd .menu li:nth-child(6n-4) ul li a{color:#ef717d}#mainHd .menu li:nth-child(6n-4) a:hover,#mainHd .menu li:nth-child(6n-4) ul li a:hover{color:#999b9a}#mainHd .menu li:nth-child(6n-3) a{color:#f49081}#mainHd .menu li:nth-child(6n-3) a:hover{color:#999b9a}#mainHd .menu li:nth-child(6n-2) a{color:#f28b2e}#mainHd .menu li:nth-child(6n-2) a:hover{color:#999b9a}#mainHd .menu li:nth-child(6n-1) a{color:#fac829}#mainHd .menu li:nth-child(6n-1) a:hover{color:#999b9a}  .menu{border-top:1px solid #ccc;clear:both;font-family:'Yanone Kaffeesatz';font-size:30px;font-weight:700;line-height:30px;list-style:none;padding-top:5px;text-align:right;text-transform:uppercase}.sb-slidebar .menu li{border-bottom:1px solid #ccc;padding:0 0 5px;margin-bottom:5px}.sb-slidebar .menu ul{list-style:none;padding-top:5px}.sb-slidebar .menu ul li{border-bottom:none;font-size:24px;line-height:24px;padding:0 0 0;font-weight:400}.sb-slidebar .menu ul li a{color:#7bd3c7}.sb-slidebar .menu ul li a:hover{color:#5fbbb6}.sb-toggle-right{display:none;cursor:pointer;float:right;margin:0;padding-top:30px;width:25px}.sb-toggle-right .navicon-line{background:#ef717d;display:block;height:5px;margin:0 auto 5px;width:25px}.sb-toggle-right:hover .navicon-line{background:#5fbbb6}#mainFoot{background:#f49081;box-sizing:border-box;clear:both;color:#fff;padding:30px 2% 0 2%;width:100%}#leftFt{box-sizing:border-box;float:left;max-width:470px;padding-right:15px;width:50%}#leftFt #ftNav .menu{color:#fff;list-style:none;float:left;border-top:6px double #fff;border-bottom:6px double #fff;font-family:'Yanone Kaffeesatz';font-size:22px;font-weight:700;line-height:22px;padding:5px 0;margin-bottom:15px;text-transform:uppercase;width:100%}#leftFt #ftNav .menu li{float:left;padding:2px 5px 2px 0;border-right:3px solid #fff;margin-right:5px}#leftFt #ftNav .menu li:last-child{padding-right:0;border-right:none;margin-right:0}#leftFt #ftNav .menu li a{color:#fff}#leftFt #ftNav .menu li a:hover{color:#f8d4cf}#leftFt .socialIcons{color:#f8d4cf;font-family:'Yanone Kaffeesatz';font-size:28px;font-weight:700;line-height:28px;list-style:none;float:left;height:33px;text-transform:uppercase;vertical-align:bottom;width:410px}#leftFt .socialIcons::before{content:"DON'T BE ANTI-SOCIAL:"}#leftFt .socialIcons li a{background-position:center bottom;background-repeat:no-repeat;display:block;height:32px;width:32px}#leftFt .socialIcons li a:hover{background-position:center top}#rightFt{box-sizing:border-box;float:right;max-width:470px;padding-left:15px;width:50%}#rightFt::after{clear:both}#rightFt #bioPic{box-sizing:border-box;float:right;height:169px;margin:0 0 10px 15px;padding:5px;width:147px}#rightFt #bioPic img{height:auto;max-width:100%}#rightFt h3{color:#fff;font-family:havana;font-size:57px;font-weight:400;line-height:57px}#rightFt h3 span{color:#fac829}#mainFoot p{line-height:18px}p.copy{clear:both;color:#fff;font-size:10px;line-height:12px;margin:0;padding:10px 0 0}.copy a{color:#606060}.copy a:hover{color:#fff}#slider{margin-bottom:30px}#recBlog{background:#f7f5ef box-sizing:border-box;padding:20px 20px 40px;margin-bottom:20px;float:right;min-height:272px;width:74%}#recBlog h2{color:#ef717d;font-family:'Yanone Kaffeesatz';font-size:34px;font-weight:700;line-height:34px;margin:0 0 5px;padding:0;text-transform:uppercase;width:100%}#recBlog p.date{font-size:12px;line-height:16px}#recBlog .more{color:#fff;float:right;font-family:'Yanone Kaffeesatz';font-size:26px;font-weight:700;line-height:26px;text-transform:uppercase}#recBlog .more a{background:#ef717d;color:#fff;display:block;padding:5px 10px;width:auto}#recBlog .more a:hover{background:#f28b2e}#newsletter{background:#ef717d;box-sizing:border-box;color:#fff;float:left;margin-bottom:20px;padding:20px 20px 50px;width:24%}#newsletter h2{font-family:havana;font-size:48px;font-weight:400;line-height:48px;margin:0;padding:0}#newsletter h3{font-family:'Yanone Kaffeesatz';font-size:24px;line-height:24px;margin:0 0 15px;text-align:right;text-transform:uppercase}#newsletter input[type=email],#newsletter input[type=text]{background:#f799a2;border:none;box-sizing:border-box;height:32px;margin-bottom:10px;padding:5px 10px;width:100%}#newsletter input[type=submit]{background:#fac829;border:none;color:#fff;font-family:'Yanone Kaffeesatz';font-size:26px;font-weight:700;float:right;letter-spacing:normal;line-height:26px;padding:5px 10px;text-transform:uppercase;width:auto}#newsletter input[type=submit]:hover{background:#b1e2dc}#pco,#shco{box-sizing:border-box;float:left;padding-bottom:40px;width:50%}#pco{padding-right:10px}#pco h3{border-top:6px double #999b9a;border-bottom:6px double #999b9a;color:#f28b2e;font-family:'Yanone Kaffeesatz';font-family:havana;font-size:44px;font-weight:400;line-height:44px;margin:0 0 15px;padding:5px 0;text-align:center;width:100%}#shco{padding-left:10px}#shco h3{border-top:6px double #999b9a;border-bottom:6px double #999b9a;color:#5fbbb6;font-family:havana;font-size:44px;font-weight:400;line-height:44px;margin:0 0 15px;padding:5px 0;text-align:center;width:100%}.releaseBlock{box-sizing:border-box;max-width:320px;float:left;height:auto;margin:0;padding:10px;width:50%}.releaseBlock img{height:auto;max-width:100%}input[type=email]{background:#d8d8d8;border:none;box-sizing:border-box;height:40px;margin:0 0 15px;padding:10px 15px;width:100%}::-webkit-input-placeholder{color:#fff}:-moz-placeholder{color:#fff}::-moz-placeholder{color:#fff}:-ms-input-placeholder{color:#fff}.rslides{box-sizing:border-box;position:relative;list-style:none;width:100%;padding:0;margin:0;z-index:0}.rslides li{-webkit-backface-visibility:hidden;position:absolute;display:none;width:100%;left:0;top:0;z-index:0}.rslides li:first-child{position:relative;display:block;float:left}.rslides img{display:block;height:auto;float:left;width:100%;border:0}@media screen and (max-width:960px){#sb-site{background:#fff}#wrapper{padding:0;width:100%}#innerWrap{padding:0 5%;width:90%}#mainHd .menu{font-size:30px;line-height:30px}}@media screen and (max-width:865px){#mainHd{margin-bottom:20px}#mainHd h1{float:none;margin:0 auto}#mainHd .menu{float:left;font-size:44px;line-height:44px;margin:10px auto;max-width:100%;width:100%}#mainHd .menu li:last-child::after{clear:both}#mainHd .socialIcons{padding:0}}@media screen and (max-width:768px){#mainHd h1{float:left;margin:0}#mainHd #searchform,#mainHd .menu,#mainHd .socialIcons{display:none}.sb-toggle-right{display:block}#leftFt .socialIcons{display:none}#leftFt,#rightFt{float:none;margin:0 auto 20px;width:100%;max-width:100%}#newsletter,#recBlog{width:48%}#pco h3,#shco h3{font-size:30px;line-height:30px}.releaseBlock{float:none;margin:0 auto;width:100%}}@media screen and (max-width:480px){#newsletter,#recBlog{float:none;width:100%}#pco,#shco{float:none;padding:0 0 30px;width:100%}}@media only screen and (-webkit-min-device-pixel-ratio:2),only screen and (min--moz-device-pixel-ratio:2),only screen and (-o-min-device-pixel-ratio:2/1),only screen and (min-device-pixel-ratio:2){#mainHd h1{-webkit-background-size:234px 163px;-moz-background-size:234px 163px;-o-background-size:234px 163px;background-size:234px 163px} @font-face{font-family:Oswald;font-style:normal;font-weight:400;src:url(http://fonts.gstatic.com/s/oswald/v31/TK3_WkUHHAIjg75cFRf3bXL8LICs1_FvsUZiYA.ttf) format('truetype')}}</style>
</head>
<body>
<div id="sb-site">
<div id="wrapper">
<div id="innerWrap">
<header id="mainHd">
<h1>{{ keyword }}</h1>
<ul class="menu" id="menu-main-navigation-menu">
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-home menu-item-1995" id="menu-item-1995"><a href="#">{{ keyword }}</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-page current_page_parent menu-item-1994" id="menu-item-1994"><a href="#">Blog</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-1998" id="menu-item-1998"><a href="#">About</a></li>
<li class="menu-item menu-item-type-taxonomy menu-item-object-category menu-item-1999" id="menu-item-1999"><a href="#">Tutorials</a></li>
</ul>
<div class="clear"></div>
</header>
{{ text }}
<p>.</p>
{{ links }}
</div>
<footer id="mainFoot">
<div id="leftFt">
<nav id="ftNav">
<ul class="menu" id="menu-footer-navigation-menu">
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-2025" id="menu-item-2025"><a href="#">Terms of Use</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-2015" id="menu-item-2015"><a href="#">Contact</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-2027" id="menu-item-2027"><a href="#">Info</a></li>
<li class="menu-item menu-item-type-post_type menu-item-object-page menu-item-2014" id="menu-item-2014"><a href="#">Additional Services</a></li>
</ul> </nav>
<div class="clear"></div>
<p class="copy">{{ keyword }}</p>
</div>
<div id="rightFt">
<div id="bioPic"></div>
<h3>{{ keyword }}</h3>
</div>
<div class="clear"></div>
</footer>
</div>
</div>
</body>
</html>";s:4:"text";s:2856:"Background CRISPR-Cas9-based technologies have revolutionized experimental manipulation of mammalian genomes. Given its ease-of-use and versatility, CRISPR is already used for a variety of applications and holds a lot of promise for the future. July 2019; The Plant Journal 100(4) ... Download full-text PDF Read full-text. <br> <br>Read on for a crash course in everything you need to know about the fundamentals of CRISPR. By delivering the Cas9 nuclease complexed with a synthetic guide RNA  (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added in vivo  (in organisms that are alive). It is based on a simplified version of the bacterial CRISPR-Cas9  antiviral defense system. A CRISPR/LbCas12a‐based method for highly efficient multiplex gene editing in Physcomitrella patens. Aims To create an optimized protocol for penetrant, reproducible, and fast targeted insertiondeletion mutation (indel) formation in cell cultures … These sequences are derived from DNA fragments of bacteriophages  that had previously infected the prokaryote. Hence these sequences play a key role in the antiviral (i.e. CRISPR technology is igniting a revolution across the life sciences and is quickly becoming a standard tool in many labs. CRISPR   (clustered regularly interspaced short palindromic repeats) is a family of DNA  sequences found in the genomes  of prokaryotic  organisms such as bacteria  and archaea. anti-phage) defense system of prokaryotes. The repeat-spacer CRISPR array contents and cas genes have undergone an extraordinarily dynamic rate of evolution leading to a complex diversity in the genetic architectures of the CRISPR-Cas systems. None-the-less, limitations of the delivery and efficacy of these technologies restrict their application in primary cells. One Sentence Summary: CRISPR-E measures a gene’s essentiality . Free Books Online PDF: CRISPR: Methods and Protocols – 2015. The CRISPR/Cas9 system is a powerful tool for genome editing in mammalian cells that allows researchers to generate genetic variants at lower cost and with higher throughput than alternative methods like zinc finger nuclease (ZFN) or transcription activator-like effector nuclease (TALEN) CRISPR gene editing is a genetic engineering  technique in molecular biology  by which the genomes  of living organisms may be modified.  Abstract: The CRISPR/Cas9 system is a powerful method of editing genes by randomly introducing errors into the target sites. CRISPR 101 eBook (Free PDF) This comprehensive eBook is the perfect resource for both scientists and non-scientists to learn the basics of CRISPR-Cas9. They are used to detect and destroy DNA from similar bacteriophages during subsequent infections. Here, we describe a CRISPR-based test for gene essentiality (CRISPR-E … ";s:7:"keyword";s:17:"crispr method pdf";s:5:"links";s:4462:"<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-danbury-sales'>Danbury Sales</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-anchorman-2-canadian-news-team-cast'>Anchorman 2 Canadian News Team Cast</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-david-shipley-spower'>David Shipley Spower</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-the-lion-and-the-mouse-story-with-pictures'>The Lion And The Mouse Story With Pictures</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-tyutnev-pavel'>Tyutnev Pavel</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-scott-stevens-stats'>Scott Stevens Stats</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-aria-restaurant-sydney-prices'>Aria Restaurant Sydney Prices</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-thames-water-vans'>Thames Water Vans</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-what-happened-to-ash-morgan'>What Happened To Ash Morgan</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-sarah-cooper-twitter%3A-how-to-medical'>Sarah Cooper Twitter: How To Medical</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-photon-os-based-on'>Photon Os Based On</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-great-keppel-island-resort-reopening'>Great Keppel Island Resort Reopening</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-soccer-laduma-orlando-pirates-news-today'>Soccer Laduma Orlando Pirates News Today</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-pay-water-charges'>Pay Water Charges</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-easy-breaks-aberdeenshire'>Easy Breaks Aberdeenshire</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-tinie-tempah-disc-overy-songs'>Tinie Tempah Disc-overy Songs</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-mexico-imf-credit-line'>Mexico Imf Credit Line</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-krc-genk-u21-vs-mechelen'>Krc Genk U21 Vs Mechelen</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-academy-of-the-holy-names-albany'>Academy Of The Holy Names Albany</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-australia-vs-germany-war'>Australia Vs Germany War</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-the-botanist-gin-lcbo'>The Botanist Gin Lcbo</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-germany-and-russia-map'>Germany And Russia Map</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-herschel-malaysia-wallet'>Herschel Malaysia Wallet</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-ec-stability-and-growth-pact'>Ec Stability And Growth Pact</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-on-that-resurrection-morning-when-the-redeemed-are-gathering-in-lyrics'>On That Resurrection Morning When The Redeemed Are Gathering In Lyrics</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-disposable-income-per-capita-by-country-2018'>Disposable Income Per Capita By Country 2018</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-severn-trent-jobs-mansfield'>Severn Trent Jobs Mansfield</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-agatha-hair-clip-amazon'>Agatha Hair Clip Amazon</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-knives-out-ending'>Knives Out Ending</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-planet-oat-milk'>Planet Oat Milk</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-evh-wolfgang-special-usa'>Evh Wolfgang Special Usa</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-egg-harbor-dispensary-menu'>Egg Harbor Dispensary Menu</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-a-year-in-space-review'>A Year In Space Review</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-restaurants-in-australia-sydney'>Restaurants In Australia Sydney</a>,
<a href='https://africarisk.net/.tmb/docs/cxqkrdv.php?id=8cc357-bureau-of-labor-statistics-consumer-price-index-calculator'>Bureau Of Labor Statistics Consumer Price Index Calculator</a>,
";s:7:"expired";i:-1;}